Chem 425s/578  Protein Engineering  Spring 2004

Literature citations given in class (* indicates assigned reading, available in reading room CB200).  The highlighted references are links to .pdf files.

 

This list will be updated periodically, so please check in here weekly.

 

 

Reading for week of 4/5-4/9:

*Chapter 17 from Branden and Tooze “Introduction to Protein Structure”

*Anthony-Cahill and Magliery, Current Pharma. Biotech., 2002, 3, 299-315

*Bilgicer and Kumar, J. Chem. Ed., 2003, 80, 1275-81

 

Other references cited in lecture:

Richardson and Richardson, Science, 1988, 240, 1648 (aa preferences in helices)

Bryson et al., Science, 1995, 270, 935 (extensive review of de novo design)

Smith and Regan, Acc. Chem. Res., 1997, 30, 153 (review of beta structure design)

Katz and Kossiakoff, J. Biol. Chem., 1986, 261, 15480 (engineering disulfides)

Matsumara et al., Nature, 1989, 342, 291 (engineered disulfides in T4L)

Mitchinson and Wells, Biochemistry, 1989, 28, 4807 (engineered disulfides in subtilisin)

 

 

Reading for week of 4/12-4/16:

*Noren et al., Science, 1989, 244, 182  (biosynthetic unnatural aa method)

*Beene et al., Biochemistry, 2002, 41, 10262  (application of unnatural aa mutagenesis)

*Camarero et al., Bioorganic Med. Chem., 2001, 9, 2479  (using inteins in vivo)

 

Other references cited in lecture:

Distefano et al., Curr. Op. Struct. Biol., 1998, 8, 459  (semi-synthesis)

Schnölzer and Kent, Science, 1992, 256, 221  (chemo-selective ligation)

Severinov and Muir, J. Biol. Chem., 1998, 273, 16205  (expressed protein ligation = EPL)

Noren, Wang and Perler, Angew. Chem. Int. Ed., 2000, 39, 450 (intein review)

Cotton and Muir, Chemistry and Biology, 1999, 6, R247 (EPL review)

Camarero and Muir, JACS, 1999, 121, 5597 (cyclic SH3 via EPL)

Iwai and Plückthun, FEBS Lett., 1999, 459, 166 (cyclic b-lactamase via EPL)

Cotton and Muir, JACS, 1999, 121, 1100 (SH3-SH2 biosensor)

 

 

Reading for week of 4/19-4/23:

*Cunningham and Wells, Science, 1989, 244, 1081  (Ala-scanning mutagenesis)

*Vogel, Cook and Chmielewski, Chemistry and Biology, 1996, 3, 295  (enzyme catalyzed peptide ligation)

 

Other references cited in lecture:

Jackson et al., Science, 1994, 266, 243  (subtiligase)

Braisted et al., Meth. Enzymol., 1997, 289, 298  (subtiligase review)

Wells, Proc. Nat’l. Acad. Sci. USA, 1996, 93, 1 (review of hGH hormone/receptor engineering)

 

 

Reading for week of 4/26-4/30:

*Smith and Petrenko, Chem. Rev., 1997, 97, 391-410  (review of phage display)

            given the length of this article it is the only assigned reading for this week

 

Other references cited in lecture:

Li et al., Science, 1995, 270, 1657 (minimization of ANP)

Lowman et al., Biochemistry, 1991, 30, 10832 (affinity maturation of hGH)

Roberts et al., Proc. Nat'l. Acad. Sci. USA, 1992, 89, 2429 (phage display of BPTI to make it a good HNE inhibitor)

Atwell et al., J. Mol. Biol., 1997, 270, 26 (remodeling protein dimmer interface)

 

 

Reading for week of 5/3-5/7:

*Dalal et al., Nature Struct. Biol., 1997, 4, 548 (solution to Paracelsus challenge)

*Cesaro-Tadic et al., Nature Biotech., 2003, 21, 679 (directed evolution of antibody catalysts)

 

Other references cited in lecture:

Braisted and Wells, Proc. Nat'l. Acad. Sci. USA, 1996, 93, 5688 (minimized Z domain)

Starovasnik et al., Proc. Nat'l. Acad. Sci. USA, 1997, 94, 10080 (optimized Z domain)

Pedersen et al., Proc. Nat'l. Acad. Sci. USA, 1998, 95, 10523 (evolution of SNase specificity)

Stemmer, Nature, 1994, 370, 389 (DNA shuffling of b-lactamase)

Crameri et al., Nature, 1998, 391, 288 (DNA shuffling with homologous genes)

Crameri et al., Nature Biotechnology, 1997, 15, 436 (pathway evolution using DNA shuffling)

 

 

Reading for week of 5/10-5/14:

*Yang et al., Nature Struct. Biol., 1997, 4, 548 (solution to Paracelsus challenge)

 

Other references cited in lecture:

Dahiyat and Mayo, Science, 1997, 278, 82 (computer aided de novo design)

Malakauskas and Mayo, Nature Struct. Biol., 1998, 5, 470 (design of more stable B1 domain)

Benson et al., Science, 2001, 293, 1641 (biosensor design)

Looger et al., Nature, 2003, 423, 185 (computational receptor design)

 

 

Reading for week of 5/17-5/21:

No required reading this week on account of Scholar’s week activities

 

Other references cited in lecture:

Bolon, Voigt and Mayo, Curr. Op. Chem. Biol., 2002, 6, 125 (de novo design of biocatalysts)

Johnsson et al., Nature, 1993, 365, 5301 (oxaldie catalytic peptides)

Tanaka and Barbas, JACS, 2002, 124, 3510 (modular catalytic peptides)

Hilvert, D., Ann. Rev. Biochem., 2000, 69, 751 (review of catalytic antibodies)

Wagner, Lerner and Barbas, Science, 1995, 270, 1797 (Aldolase catalytic antibody)

Rader et al., Proc. Nat'l. Acad. Sci. USA, 2003, 100, 5396 (extending half-life of small drugs by conjugation to aldolase antibody)

Quéméneur et al., Nature, 1998, 391, 301 (converting cyclophilin to an endopeptidase)

Lazar et al., Curr. Op. Struc. Biol., 2003, 13, 513 (designing proteins for therapeutic applications)

Luo et al., Protein Science, 2002, 11, 1218 (design of G-CSF with improved stability)

Kochendoerfer et al., Science, 2003, 299, 884 (synthetic, polymer-modified EPO)

Liu et al., Proc. Nat'l. Acad. Sci. USA, 2003, 100, 657 (engineered anthrax toxin)

Root, Kay and Kim, Science, 2001, 291, 884 (HIV-1 entry inhibitor design)

Bewley et al., J. Biol. Chem., 2002, 277, 14238  (peptide inhibitor of HIV-1 fusion)

 

 

Reading for the remainder of the course (5/24-6/4):

*The four papers presented by student groups are REQUIRED reading, you can get the articles at the following URL:

http://atom.chem.wwu.edu/sacahill/425/group_assignments_S04.htm